Coding

Part:BBa_K1968024:Design

Designed by: Ricardo Camilo Chávez Martínez   Group: iGEM16_Edinburgh_OG   (2016-10-14)


LacI without LVA-tag (Repressor) Phytobrick


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed under the Phytobrick standard. This part had to be modified in order to adjust to the Phytobrick standard. Two BbsI sites had to be removed (Codon in the basepair 494-496 was changed from GAT to GAC, the codon in basepair 962-964 was changed from GTC to GTG maintaining the same aminoacid sequence and making it Phytobrick compatible)


Source

Based on the part BBa_K1088018. This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing

References